2024 Alk cell line

Alk cell line

Author: gaeh

August undefined, 2024

WebAn isogenic cell line was created to model cancer patients with the echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) fusion oncogene, a key oncogenic driver, and then tested for its sensitivity to known selective inhibitors of ALK. INTRODUCTION WebH3122, an ALK -translocated cell line, was exposed to ALK TKI (TAE) or a combination of PI3K and MEK inhibitors (ZS+CI) or both. (A) phase contrast micrograph of the cells when exposed to the ...

Iruplinalkib (WX‑0593), a novel ALK/ROS1 inhibitor, …

WebApr 4, 2024 · Cell lines with ALK alterations were in general less sensitive to lorlatinib than to ceritinib and other ALK inhibitors based on growth inhibition assays (Fig. 1A i–v) which … WebFeb 28, 2024 · The characteristics of each cell line, including EML4-ALK fusion protein expression and IC 50 values of ALK-TKIs (alectinib, ceritinib, lorlatinib, and brigatinib) … unc healthcare management

ALK inhibitor and ALDH1 in the H3122 NSCLC line. H3122, an ALK ...

WebJul 1, 2008 · Purpose: The EML4-ALK fusion gene has been detected in ∼7% of Japanese non-small cell lung cancers (NSCLC). We determined the frequency of EML4-ALK in Caucasian NSCLC and in NSCLC cell lines. We also determined whether TAE684, a specific ALK kinase inhibitor, would inhibit the growth of EML4-ALK-containing cell lines … WebAnaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, … WebMar 28, 2024 · Kaplan-Meier estimates of PFS are also shown for those ALK-positive, evaluable patients who were ALK TKI-naïve (red line), received prior crizotinib only (green line), and received prior crizotinib and a 2nd generation inhibitor (blue line), where the median PFS for those groups was 26.2 months, 9.0 months, and 1.9 months, … unc healthcare mba

Cell line - ALK - The Human Protein Atlas

APPLICATION NTE - ATCC

WebIn ALK-rearranged NSCLC cell lines, ganetespib induced loss of EML4–ALK expression and depletion of multiple oncogenic signaling proteins in ALK-driven NSCLC cells, … WebAn isogenic cell line was created to model cancer patients with the echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) fusion oncogene, a … thorp engineeringWebApr 14, 2024 · Abstract. High-risk neuroblastoma (NB) has a poor prognosis despite multimodal treatment. To improve survival and minimise treatment side-effects, research has focused on developing more effective therapeutic strategies. Anaplastic lymphoma kinase (ALK) is a promising druggable target as its expression rapidly decreases in healthy … thorpe north western

"WebProkaryotic Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of NPM-ALK transcript (419-520 aa). Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or … " - Alk cell line

Alk cell line

NPM-ALK Is a Key Regulator of the Oncoprotein FOXM1 in ALK ... - PubMed

WebNovel human-derived EML4-ALK fusion cell lines identify ribonucleotide reductase RRM2 as a target of activated ALK in NSCLC. We provide a characterization of four … WebAnaplastic lymphoma receptor tyrosine kinase (ALK), also known asp80, is a receptor tyrosine kinase first identified as the protein product of the translation of an abnormalALK - NPM fusion gene produced by a t (2;5) (p23;q35) chromosomal translocation in anaplastic large cell lymphoma (ALCL).257 Corresponding overexpression of ALK that can be ...

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WebJan 2, 2007 · In this work, we developed a cell proliferation assay using NPM-ALK-transformed murine pre-B cell line (Ba/F3) to identify a highly potent and selective inhibitor of ALK kinase activity, NVP-TAE684. TAE684 blocked proliferation and survival of Ba/F3 NPM-ALK, SU-DHL-1 and Karpas-299 cells with 50% inhibitory concentrations (IC 50 ) … WebApr 4, 2024 · Cell lines with ALK alterations were in general less sensitive to lorlatinib than to ceritinib and other ALK inhibitors based on growth inhibition assays (Fig. 1A i–v) which we believe is due to the presence of a concomitant RAS-MAPK pathway mutation in cell lines which were hyperresistant to lorlatinib (except for cell line NB1691, which has ...

WebFeb 24, 2024 · Fig. 1: Identification of gilteritinib as an inhibitor of ALK-rearranged cancer cells. a Relative cell viability of parental (with IL-3), EML4-ALK wild-type (WT), EML4 … WebThe cell line was designated ABC-11. The EML4-ALK fusion protein and genes were identified in ABC-11 cells using fluorescence in situ hybridization and …

WebIn ALK-rearranged NSCLC cell lines, ganetespib induced loss of EML4–ALK expression and depletion of multiple oncogenic signaling proteins in ALK-driven NSCLC cells, leading to greater in vitro potency, superior antitumor efficacy, and prolonged animal survival compared with crizotinib monotherapy. 19,20 Ganetespib also overcame multiple forms ... WebCell line: Ba/F3 is a pro-B murine cell line dependent on interleukin-3 (IL-3) for growth. It is a classical cell line for investigations of cytokine receptors. It does not endogenously express the PRLR, and is absolutely dependent on cytokine receptor signaling for …

WebMar 1, 2003 · A direct role for NPM-ALK in cellular transformation has been shown in vitro with immortalized cell lines and in vivo using retroviral transfer experiments. Nonetheless, there is no direct evidence of its oncogenic potential in T lymphocytes, which represent the most common target of ALK chimeras.

WebEach slide contains two, 2mm diameter cell line cores which demonstrate positive and negative expression for the specific marker. APPLICATION: Newcomer Supply ALK-Lymphoma (anaplastic lymphoma kinase) Cell Line Control Slides are for the positive immunohistochemical staining of ALK-Lymphoma with a NPM (nucleophosmin) gene … thorpe northamptonshireWebApr 5, 2024 · Endoglin is expressed by activated endothelial cells acting as a co-receptor for the TGF-β superfamily and is essential for angiogenesis. 36 ALK-1, which is an endothelial specific TGF-β type I receptor, interacts with endoglin to promote endothelial proliferation, but in contrast when associated with ALK-5, endoglin suppresses proliferation ... thorpe norfolkWebOct 16, 2008 · Analysis of the ALK gene in a panel of 30 neuroblastoma cell lines revealed sequence variants in 6, including 3 different cell lines containing the F1174L mutation … unc healthcare my timeWebAug 6, 2024 · Inhibition of FOXM1 in two NPM-ALK + ALCL cells using shRNA and pharmalogic agent (thiostrepton) resulted in reductions in cell growth and soft-agar colony formation, which were associated with apoptosis and cell-cycle arrest. FOXM1 is functionally linked to NPM-ALK, as FOXM1 enhanced phosphorylation of the NPM-ALK/STAT3 axis. unc health care middlesex ncWebApr 3, 2024 · Compared with the median of all 37 cell lines, mRNA levels at the C-terminus of all five of the fusion-positive cell lines tested (three ALK, one RET, and one ROS1) were elevated at least 2000-, 300-, or 2000-fold, respectively, and high C-terminal protein expression was detected. In an ALK fusion–positive tissue specimen, the mRNA and ... unc healthcare morgantonWebEML4-ALK Fusion-A549 is an isogenic cell line created at ATCC by using CRISPR/Cas9 genome-editing technology. CCL-185IG contains EML4-ALK fusion variant 1 (E13; A20). … thorpe north \\u0026 western llpWebJul 2, 2015 · Systemic anaplastic large cell lymphoma (ALCL) is an aggressive CD30 + non-Hodgkin lymphoma. Anaplastic lymphoma kinase–positive (ALK+) ALCL is associated with the NPM-ALK t(2;5) translocation, which is highly correlated with the identification of the ALK protein by immunohistochemistry. ALK+ ALCL typically occurs in younger patients … thorpe north \\u0026 western