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WebSep 1, 2024 · Here, we report a new workflow named VHUT-cryo-FIB (Vibratome–High-pressure freezing–Ultramicrotome Trimming–cryo-FIB) to prepare cryo-tissue lamellae for high-quality cryo-ET data collection (Supplementary Movie 1). The whole workflow included fresh tissue sample slicing using a vibratome, HPF, pretrimming by a cryo … WebDec 18, 2024 · Cryo-ultrathin sectioning of the epicarp can be difficult due to various factors, such as the water content of the specimen, the amount of frozen flesh and excess juice, and the size of the cutting surface. Trimming out most of the frozen flesh and juice improves the homogeneity of mechanical properties of the specimen. builder hampshire
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