Hct116 puromycin
WebMar 25, 2024 · Next, we constructed knockout plasmid by cloning the sgRNA into the pCasCMV-Puro-U6 vector. After transfection of the plasmid into HCT-116 cells, TrxR1 knockout HCT-116 cells were selected using puromycin resistance. The TrxR1 knockout efficiency was identified and verified by DNA sequencing, immunoblotting, TRFS-green … WebHCT116 knockout cells were infected with a blank or a p53-expressing lentivirus; HCT116 parental p53 wt cells were infected with a blank …
Hct116 puromycin
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WebDNR LBRU Rev 7-20-20 NOTIFICATION OF SALE, THEFT, RECOVERY, DESTRUCTION OR ABANDONMENT OR MOVED FROM STATE FOR A GA REGISTERED VESSEL … WebFeb 22, 2010 · For IL4Ra knockdown in HCT116 cells, ‘Sure-Silencing’ plasmids encoding shRNA sequences directed toward four different regions of human IL4Ra ... murine or human cells were placed under selection with puromycin (10 μg/ml) or hygromycin (200 μg/ml), respectively. Once stable populations were obtained, individual clones were …
WebSPOLIATION OF EVIDENCE From the Georgia Bar Journal By Lee Wallace The Wallace Law Firm, L.L.C. 2170 Defoor Hills Rd. Atlanta, Georgia 30318 404-814-0465 WebHCT116-Fluc-Puro is a polyclonal population of the human colorectal carcinoma cell line HCT116 (ATCC® CCL-247™). To achieve stable reporter expression in the polyclonal population, parental HCT116 cells …
WebThis Luciferase & GFP / human HCT116 cell line was transformed via Lentivirus transduction. It has the Luciferase and GFP was constitutively co-expressed under EF1a promoter, as two separate proteins (not as fusion), mediated via 2A element. The Puromycin resistance marker was expressed under Rsv promoter. The following … WebApr 12, 2024 · Puromycin was added to the cells for 5 days and individual cell colonies were isolated. The knockout effect was examined by Western blot with DNMT1 antibody (clone EPR3521(2); Abcam) in the ...
WebPuromycin titration in HCT116 cells. Cells were treated with given concentrations of puromycin. Cell death was microscopically monitored over time. Scale bar: 50 µm. 4 x …
WebApr 30, 2024 · To obtain cell lines stably overexpressing the lncRNA Ftx, HCT116 cells were transfected with Ftx and screened with puromycin (2 μg/mL), whereas HCT116 cells transfected with Ftx-NC were used as its mock control. The stable lncRNA Ftx-interfered or -overexpressing cell lines were validated by real-time PCR with the following primers … hop n play hoursWebGeneticin (G418 sulfate), zeocin, hygromycin B, puromycin, and blasticidin antibiotics are the most common selection antibiotics used for stable cell transfection. These antibiotics provide unique solutions for your research needs, such as dual selection and rapid, stable cell line establishment. Explore all Gibco selection antibiotics long war of the chosen downloadWebPuromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. This antibiotic inhibits the growth of Gram-positive bacteria and various animal and insect cells. long war of the chosen modsWebHCT116-hNIS-Neo/Fluc-Puro is a polyclonal population of the human colorectal carcinoma cell line HCT116 (ATCC® CCL-247™). To achieve stable reporter expression in the polyclonal population, parental HCT116 cells were transduced with LV-hNIS-Neo (LV013) and LV-Fluc-P2A-Puro (LV012) and selected using G418 and puromycin. long war of the chosen classes guideWebDec 30, 2024 · Puromycin is used for selection and maintenance of cell lines expressing a transfected pac gene (S. alboniger), whose product, puromycin acetyltransferase, inactivates puromycin via acetylation; recommended concentration in cell culture 1-10µg/ml. Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of … long war of the chosen githubWebHCT116 cells have been widely used in colorectal cancer research. HCT116/GFP (CMV) stable cells express GFP under the control of the CMV promoter. ... Puromycin: Biosafety Level: BSL-2: Shipped: Dry ice : Storage: Liquid Nitrogen: Catalog Number Product Size Price Qty; SC-1001. HCT 116/GFP (CMV) stable cells. 2x10^6 cells: Price: Quantity. … hopnwhichWebSep 16, 2024 · To examine the role of CTRP1 in tumor progression, we generated CTRP1 knockout A549 and HCT116 cell lines, which reduced the expression levels of nuclear factor (NF)-κB-dependent and metastasis-promoting transcripts. ... and cells were selected with puromycin (2 µg/mL). Control cells were generated by transduction of an empty vector in ... long war of the chosen liberation