In a tabletop microcentrifuge
WebApr 12, 2024 · Centrifuge all tubes at 2500 × g for 2 min in the Spectrafuge 24D microcentrifuge. Repeat for a total of two washes. 15. Remove supernatant after the second wash. Refer to Table 2 to calculate the total resuspension volume based on the tests ordered. A PBMC/BMMC aliquot is always included per sample. WebThe remaining mixture of worms was transferred to a low-bind RNAse-free microcentrifuge tube and centrifuged at 1.6 rpm for 2 min. After this centrifugation cycle, as much supernatant as possible was removed without disturbing the worm pellet. To the pellet, 500 μL of Trizol reagent was added and vortexed for 30 s. ... (Table 1 and Table 2).
In a tabletop microcentrifuge
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WebApr 11, 2024 · Different nutritional regimens have been reported to exert beneficial effects on obesity through the regulation of the composition and function of gut microbiota. In this context, we conducted in obese subjects two dietary interventions consisting of a low-calorie and two-phase (ketogenic plus low-calorie) diet for 8 weeks. Anthropometric and clinical … WebBriefly spin down the PCR tubes (2-3 s) using a tabletop microcentrifuge in order to ensure that all of the reagents are in the reaction mixture Place the PCR tubes into the selected thermocycler. Once the lid to the thermocycler is properly closed, start the required amplification program, as in table 3. Table 3: Phusion Amplification protocol
WebSep 18, 2014 · “ Microcentrifuges ” are those in which 1.5-mL tubes are typically spun, whereas in “general purpose” (GP, or sometimes just plain “centrifuge”) units you’re looking at mostly 15- and 50-mL conical tubes. Web• All centrifugations should be carried out in a table-top microcentrifuge at >12000 ××× g (10 000-14 000 rpm, depending on the rotor type). Use 1-5 mL of E. coli culture in LB media for purification of high-copy plasmids. For low-copy plasmids use up to 10 mL of culture. Protocol A. Plasmid DNA purification using centrifuges Step Procedure 1
Webmicrocentrifuge Air-cooled 24-place micro-centrifuge for basic molecular biology ap-plications Whisper quiet 24-place standard microcentrifuge with outstanding feature set for broad range of applications, refrigerated for temperature sensitive samples or non-refrigerated High-throughput refrigerated 48-place micro-centrifuge Crossover microcentri- Web6. Centrifuge the lysate for 10 min at maximum speed in a tabletop microcentrifuge (13,000 rpm or ≥10,000 x g). 7. Meanwhile, place a QIAprep Spin Column in a 2 ml collection tube. 8. Transfer the cleared lysates from step 6 to the QIAprep Spin Column by decanting or pipetting. 9. Centrifuge 30–60 s (13,000 rpm or ≥10,000 x g). Discard ...
WebJan 1, 2015 · Centrifuge at 16,000 × g for 1 min at room temperature in a tabletop microcentrifuge. 6. Remove supernatant and resuspend the pellet in 300 μl buffer P1 containing RNase A. Mix well by pipetting up and down until no cell clumps remain ( see Note 10 ). 7. Add 300 μl buffer P2 and mix gently by inverting the tube 4–6 times ( see …
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