Mcherry monoclonal antibody
WebJul 6, 2024 · After that, mCherry-tagged HA 2 subunit with NTD, IMD-CIL, and CIL-TMD fragments was expressed in HEK293T cells, and the expression of the interest proteins was detected with anti-mCherry or EGFP antibodies by Western blotting (Fig. 5 C and D). WebUsing western blot with suck reagents such as mcherry antibodies, aox antibodies , describe an experiment to show that peroxisomes are being degraded by autophagy not proteasome ... their levels should be decreased upon MG132 treatment but increased upon bafilomycin A1 treatment. Antibodies against AOX or other peroxisomal proteins can be used ...
Mcherry monoclonal antibody
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WebAnti-mCherry antibody [EPR20579] (ab213511) Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by recombinant technology for fast production Success from the first experiment – confirmed specificity through extensive validation WebThermo Fisher Scientific's mCherry Antibody is a Rat monoclonal antibody. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blot. Immunofluorescent analysis of mCherry Tag was performed using 70% confluent mCherry-H3 transfected …
WebAll mCherry antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein. Application Note. Polyclonal anti-mCherry is designed to detect mCherry, RFP, and its variants. Anti-mCherry (Discosoma sp.) has been tested by ... WebAnti-mCherry Antibody, clone IC51, Cat. No. MAB131873 detects mCherry protein and is tested for use in Immunocytochemistry and Western Blotting. Western Blotting Analysis: A representative lot detected mCherry in Western Blotting applications (Krajcovic, M., et. al. (2013). Mol Biol Cell. 24 (23):3736-45).
WebProduct Description. Mouse anti mCherry antibody recognizes the red fluorescent mCherry protein, a monomeric engineered derivate of a fluorescent protein isolated from members of the Discosoma (mushroom coral) family. mCherry is a second generation fluorescent protein and by far the most popular red emitting fluorescent protein due to its high ... WebApr 15, 2024 · Primary antibodies listed below were used in this study: Monoclonal ANTI-FLAG M2 antibody (Cat# F1804, Sigma, 1:2000), Anti-mCherry antibody [1C51] (Cat# ab125096, Abcam, 1:2000) and HRP ...
WebHowever our alternate mouse monoclonal antibody to mCherry, MCA-1C51, binds equally well to mCherry and Tomato. A sequence alignment of mCherry and GFP with epitope information is here. MCA-5A6 antibody can be used to verify the size of fusion constructs by western blotting, and to amplify the endogenous fluorescence of mCherry in transfected ...
WebHuman mCherry Alexa Fluor® 750-conjugated Antibody Recombinant Monoclonal Antibody. FAB11041S Flow Show Size & Price Human mCherry Alexa Fluor® 594-conjugated … ramblers societyWebmCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic … ramblers solo holidaysWebBackground Information. mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to … ramblers scottish paths mapWebmCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making … ramblers soccerWebThis antibody does not cross-react with green fluorescent protein. Recombinant mCherry protein, expressed in and purified from E. coli. Affinity purification. Supplied in Phosphate Buffered Saline with 20% Glycerol and 0.05% Sodium Azide. overflow inkontinans nedirWebRed fluorescent proteins (RFPs) have broad applications in life science research, and the manipulation of RFPs using nanobodies can expand their potential uses. However, the structural information available for nanobodies that bind with RFPs is still insufficient. In this study, we cloned, expressed, purified, and crystallized complexes formed by mCherry with … overflow inletWebAnti-mCherry Antibody Products. The following lists a number of MCherry antibodies from various sources. The target antigen refers to the fluorescent protein tag, mCherry, which can be attached to recombinant proteins for detection. The mCherry protein consists of 236 amino acid residues with a mass of 26.7 kilodaltons. To search for specific ... overflow inlet翻译