Ovcar3细胞是什么细胞
Web摘要:目的 检测溶酶体跨膜蛋白5(LAPTM5)在人卵巢腺癌细胞(OVCAR3)中的表达, 观察LAPTM5对OVCAR3细胞迁移、侵袭能力的影响, 并探讨LAPTM5在上皮-间质转化(EMT)过程中的作用。方法 采用Western blotting检测OVCAR3中LAPTM5的表达情况; 将OVCAR3细胞随机分为2组, 分别转染空载质粒和LAPTM5沉默质粒, 实时定量PCR检测转染 ... http://html.rhhz.net/ZGYKDXXB/html/2024/8/2024-8-678.htm
Ovcar3细胞是什么细胞
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http://www.crisprbio.net/xibao/rxb/3675.html WebNational Center for Biotechnology Information
http://www.biofeng.com/xibao/xibaozhu/NIH:OVCAR-3.html WebMar 30, 2024 · 附图说明 35.下面结合附图和实施例对本发明做进一步的说明,其中: 36.图1为本发明实施例中car-nk细胞构建的技术路线图; 37.图2为本发明实施例1中细胞和组织的鉴定结果图,其中,a为nk细胞的流式检测cd3-cd56 + 鉴定图,b为细胞ovcar3、skov3高表达间皮素的荧光成像图,c为原代卵巢癌肿瘤组织高表达间 ...
Web细胞培养步骤:. 1)复苏细胞:将含有1mL细胞悬液的冻存管在37℃水浴中迅速摇晃解冻,加入5mL培养基混合均匀。. 在1000RPM条件下离心5分钟,弃去上清液,补加4-6mL完全培养基后吹匀。. 然后将所有细胞悬液加入培养瓶中培养过夜(或将细胞悬液加入6cm皿中 ... WebJul 8, 2024 · In contrast, in HERV-K env KO OVCAR3 cells, the level of phospho-RB protein was significantly reduced, but other protein levels were not changed. The results of this study showed that HERV-K env gene KO affects cell proliferation, invasion, and migration of ovarian cells through RB and Cyclin B1 proteins, but the specific regulation pattern can …
Webanalysis of NIH-OVCAR3 highlights the complexity of the molecular profile of ovarian cancer and the di culties in predicting sensitivity using a single or even multiple molecular determinants. 2. Results 2.1. NIH-OVCAR3 Cells Are Representative of HGSOC, with TP53 Mutation, a Low Number of Mutations and High Frequency of Copy Number Alterations
Web人卵巢癌细胞系(nih:ovcar-3)是 1982 年从一名卵巢进行性腺癌患者的恶性腹水中分离出来的上皮细胞。nih:ovcar-3细胞系的科研应用广泛,常用于3d细胞培养的研究。 title companies in wilmington ohioWeb一、原理. 细胞凋亡中, 染色体DNA双链断裂或单链断裂而产生大量的粘性3'-OH末端,可在脱氧核糖核苷酸末端转移酶(TdT)的作用下,将脱氧核糖核苷酸和荧光素、过氧化物酶、碱性磷酸酶或生物素形成的衍生物标记到DNA的3'-末端,从而可进行凋亡细胞的检测,这 ... title companies in winter havenWebAug 4, 2024 · We tested a panel of tyrosine kinase inhibitors for the ability to sensitize resistant ABCB1-overexpressing ovarian cancer cell lines to paclitaxel. We observed synergy when we combined poziotinib or lapatinib with paclitaxel in resistant TOV-21G and OVCAR3 cells. Silencing ABCB1 expression in paclitaxel-resistant TOV-21G and OVCAR3 cells ... title companies in winston salem ncWebJun 8, 2024 · Increased expression level of miR-20a contributes to cisplatin-resistance of OVCAR3/DDP cells. It was reported that miR-20a was involved in drug-metastasis in various types of cancer cells (16,20).In order to investigate whether miR-20a performs a role in cisplatin-resistant OVCAR3 cells, mRNA expression levels of miR-20a in OVCAR3 and … title companies in zephyrhills flhttp://www.yajimall.com/product/detail/10297.html title companies kingsport tnWeb1)取0.1mL的浓盐酸 加入12mL的纯水中,所得到溶液的pH≈1. 2)取pH≈1的稀盐酸1-2mL 溶解100mg的牛胰岛素,一般情况下2-3分钟胰岛素能溶解(期间使用移液器轻轻吹打能加速溶解过程。. ). 3)溶解好后向溶液中加入纯水使溶液的体积为10mL(此时溶液pH≈2)。溶液 … title companies kenosha wiWebDec 10, 2024 · OVCAR3细胞源自一名60岁白人女性卵巢肿瘤组织,由T.C.Hamiltonyu建系于1982年。该细胞带有雌、雄激素受体。对阿霉素,顺氯氨铂,(左旋)苯丙氨酸氮芥有一定抗药性,适用于卵巢癌的抗药性研究。OVCAR-3染色体数量在亚三倍体范围内。 title companies lakeport ca