WebMay 1, 2002 · Here we describe the construction and application of six new tagging vectors allowing the fusion of two different types of tagging sequences, epitope and localization tags, to any Bacillus subtilis protein. These vectors are based on the backbone of pMUTIN2 and replace the lacZ gene with tagging sequences. Fusion of the tagging sequences occurs … WebNov 1, 1998 · SUMMARY: To study the functions of the uncharacterized open reading frames identified in the Bacillus subtih genome, several vectors were constructed t o …
The influence of YutF production on yutF expression in strains …
WebGTCCC-39) and subsequent cloning in pMutin2. pMutin2-MDL was constructed by PCR amplification of an internal fragment of the lsp gene with the primers lsp-m5 (59 … Web10 hours ago · Vladimir Putin's elite special forces have been all but wiped out on the battlefield in Ukraine, leaked documents reveal.. The Russian President's Spetsnaz forces have allegedly been left ... お腹 締め付け 苦しい
NhaK, a novel monovalent cation/H - Springer
WebpMutin2: pBR322-based integration vector for B. subtilis; contains a multiple cloning site downstream of the P spac promoter and a promoterless lacZ gene preceded by the RBS … WebDec 22, 2009 · B. subtilis disruption mutant strains yclNd (yclN::pMUTIN2), yclPd (yclP::pMUTIN2), and yclQd (yclQ::pMUTIN2) in the strain 168 background were obtained from E. Dervyn (Institut National de la Recherche Agronomique, Paris) . Iron-limited medium was prepared as described previously . Siderophores were obtained as described … WebFeb 22, 2015 · Background The two-component regulatory system, involving the histidine sensor kinase DegS and response regulator DegU, plays an important role to control various cell processes in the transition phase of Bacillus subtilis. The degU32 allele in strain 1A95 is characterized by the accumulation of phosphorylated form of DegU (DegU-P). Results … お腹 腰 赤い斑点